Basic Information:

1. General Information of Requester

Daping Hospital ＆ Research Institute of Surgery, a comprehensive medical institution combining a large general hospital with a special research institute, owns advanced research platform represented by one national key laboratory of trauma, burn and combined injury. We have undertaken many scientific research projects including National “973” Program, National “863” Program, Scientific and Technological Support Programs, and the National Natural Science Foundation of the major and key projects, awarded a lot of achievements such as the first prize of National Scientific and Technological Progress, and meanwhile had a series of high-level research papers published. The department of laboratory medicine devoted to the research of application of new functional nanocomposites and biosensors.

2. International Cooperation Status

At present, the research group has cooperated with the research group of Professor Luo Dan of Cornell University in the field of functional DNA nanotechnology. The two sides expect to build a joint R & D laboratory based on functional DNA nanotechnology.

Technical Requirements Summary:

1. Importance and necessity of requested project, and relations with key projects and industries of Chongqing .

The detection of circulating tumor cells（CTCs）is significant for the early diagnosis, prognosis, and efficacy evaluation of tumer metastasis. Under the previous study of the surface acoustic wave sensor and inspired by the functional DNA self-assembly, we want to establish a novel ultrasensitive acoustic resonators using functional DNA nano-probes. We introduce physical acoustic resonators to biological detection field. In order to ensure the stability of this biosensors, we plan to design a new insert type chip and corresponding micro-capacity detection system. Furthermore, the hyperbranched DNA are autonomously assembled in the surface of biosensor through a nonlinear hybridization chain reaction. The precise control of hyperbranched DNA is used to specificilly detect CTCs. Finally, a novel ultrasensitive acoustic resonators is established for CTCs detection. The present study aims to provide new method for earlily identifing the tumer metastasis and investigating the mechanisms.

2. Technology breakthroughs and innovations of requested project

A novel ultrasensitive acoustic resonators is established for CTCs detection by the angle of physics sensing technology. The technique provides new method for earlily identifing the tumer metastasis and investigating the mechanisms.

A rapid and high-specific approach for detection of CTCs was constructed by acoustic resonators. This technique combined the sensitivity of acoustic resonators with the specificity of hyperbranched DNA.