Technical Requirements Summary:

1. Importance and necessity of requested project, and relations with key projects and industries of Chongqing .

At present, the incidence and mortality of cancer remain high. Traditional treatment has already reached the bottleneck stage. The immunotherapy of tumor is currently the most promising way of treatment. A lot of remarkable results suggested that the modification of T-cells with CAR(CAＲ-T) could be a powerful approach for developing safe and effective cancer therapeutics. A novel breakthrough has been made in CAR-T treatment of hematologic tumors and therapeutic potential in clinical studies of solid tumors has been achieved. But there are still many problems for CAR-T on the clinical application such as off-target effects, cytokine storm, insertion mutagenesis, limited effects for solid tumors. It is very important for CAR-T target immunotherapy to reduce side-effect. This study is designed to treat tumors by targeting the tumor site through an in vitro CAR-T, designed by gene engineering.

2. Technology breakthroughs and innovations of requested projectCAR-T is activate by light in vitro; the technology is currently blank in the world.